Simplified Estimation of Calcium in Biological Materials by Flame Photometry
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چکیده
منابع مشابه
The determination of magnesium in biological materials by flame photometry.
A method is described for the measurement of magnesium in biological materials and has been applied to the Unicam flame spectrophotometer SP 900. Samples were diluted in 10 mM strontium ethylenedinitrolo tetraacetate (EDTASr), 10 mM potassium carbonate, giving final magnesium concentrations of about 0.1 to 0.2 mM. The EDTASr almost eliminated the depression of magnesium emission by interfering ...
متن کاملThe determination of calcium in biological fluids by flame photometry.
The use of flame photometers has greatly simplified and expedited the estimation of sodium and potassium. Calcium is one of the elements which it is possible to determine by flame photometry, but little advantage has been taken of this fact in biological work for a number of reasons. The concentration of calcium in extracellular fluids is usually less than that of potassium ; a given quantity o...
متن کاملA new principle applied to the determination of calcium in biological materials by flame photometry.
The effect of magnesium sulphate in releasing calcium emission from interference by phosphate and sulphate has been investigated. Samples were diluted in 10 mM MgSO(4), 2 mM NaCl, giving final calcium concentrations of about 0.05 to 0.10 mM. In this diluent, galvanometer readings were proportional to calcium concentrations up to 0.4 mM. The magnesium sulphate released calcium emission from depr...
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Received for publication 12 July 1968. ashed in a muffle furnace at 600'C for at least three hours. Of the acid ammonium sulphate, 10 ml, and 30 ml of water are added to the ashed sample, and after warming to dissolve soluble material the contents are decanted with washing into a 50 ml centrifuge tube, the BaSO4 being washed into the tube as far as possible. The tube is centrifuged for 5 min, t...
متن کاملThe estimation of calcium in urine by flame photometry, with a note on the estimation of sodium and potassium.
Many methods for calcium estimation by flame spectrophotometry have been described. Investigations of biological material have dealt chiefly with plasma (Baker, 1955; Chen and Toribara, 1953; Denson, 1954; Kingsley and Schaffert, 1953; Llaurado, 1954; Maclntyre, 1954; Mosher et al., 1951; Rothe and Sapirstein, 1955; Winer and Kuhns, 1953; Zak et al., 1953), with application to urine in some cas...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1954
ISSN: 0021-9746
DOI: 10.1136/jcp.7.2.110